Composite
SNAT

Part:BBa_K2276003:Design

Designed by: Zhiwei Luo   Group: iGEM17_SCU_China   (2017-10-22)


T7 promoter and the coding sequneces of serotonin N-acetyltransferase (SNAT) with N-His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 804
    Illegal XhoI site found at 796
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 264
    Illegal NgoMIV site found at 618
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized codon of SNAT in order to improve the expression that codon bias was one of the important parameter which influence gene expression.


Source

The original sequnence of tryptophan decarboxylase (TDC) comes from GenBank (accession number U29663). The sample used for experiments was artificially synthesized by Tsingke.

References

Byeon Y, Back K. Melatonin production in Escherichia coli, by dual expression of serotonin N -acetyltransferase and caffeic acid O -methyltransferase[J]. Applied Microbiology & Biotechnology, 2016, 100(15):6683.